Artificial oocyte activation in intracytoplasmic sperm injection cycles using testicular sperm in humanin vitrofertilization

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Artificial oocyte activation in intracytoplasmic sperm injection cycles using testicular sperm in human in vitro fertilization

OBJECTIVE Artificial oocyte activation (AOA) is an effective method to avoid total fertilization failure in human in vitro fertilization-embryo transfer (IVF-ET) cycles. AOA performed using a calcium ionophore can induce calcium oscillation in oocytes and initiate the fertilization process. We evaluated the usefulness of AOA with a calcium ionophore in cases of total fertilization failure in pr...

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Artificial oocyte activation with calcium ionophore A23187 in intracytoplasmic sperm injection cycles using surgically retrieved spermatozoa.

OBJECTIVE To evaluate the effect of artificial oocyte activation (AOA) on intracytoplasmic sperm injection (ICSI) cycles using surgically retrieved sperm. DESIGN Laboratory study. SETTING Fertility/assisted fertilization center. PATIENT(S) Couples undergoing surgical sperm retrieval for ICSI (n = 204). INTERVENTION(S) Application of calcium ionophore A23187 for AOA. MAIN OUTCOME MEASU...

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Endometriosis Affects Oocyte Morphology in Intracytoplasmic Sperm Injection Cycles?

OBJECTIVE To identify associations between presence of endometriosis and oocyte defects, embryo developmental potential, and cycle outcomes. METHODS This study looked into the impact of endometriosis on oocyte and embryo quality, and blastocyst formation probability. Endometriosis was also correlated with cycle characteristics. In order to avoid age-related bias, in the first analysis only pa...

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Testicular versus epididymal spermatozoa in intracytoplasmic sperm injection treatment cycles.

BACKGROUND Normal fertilization and ongoing pregnancy can be achieved using intracytoplasmic sperm injection (ICSI), even with severely immature spermatozoa. However, the published literature documents conflicting results as to the outcome of ICSI. METHODS Surgical extraction of spermatozoa in 111 ICSI treatment cycles performed over five years at the Assisted Conception Unit (ACU), Universit...

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Sperm-induced oocyte activation in the rhesus monkey: nuclear and cytoplasmic changes following intracytoplasmic sperm injection.

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ژورنال

عنوان ژورنال: Clinical and Experimental Reproductive Medicine

سال: 2015

ISSN: 2233-8233,2233-8241

DOI: 10.5653/cerm.2015.42.2.45